Viral load of various tissues of rainbow trout challenged with viral haemorrhagic septicaemia virus at various stages of disease
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Viral load of various tissues of rainbow trout challenged with viral haemorrhagic septicaemia virus at various stages of disease.
Market-sized rainbow trout Oncorhynchus mykiss were challenged by waterborne exposure to viral haemorrhagic septicaemia virus (VHSV isolate of genogroup Ia). Fish were sampled at 4 stages of infection (before onset of clinical signs, clinically affected fish, mortalities and survivors) and the viral load determined in (1) internal organs, (2) muscle tissue and (3) brain and gill tissue. Virus l...
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Cells isolated from rainbow trout fins Oncorhynchus mykiss, Walbaum were propagated and subcultured a s fibroblast-like. Viral haemorrhagic septicaemia virus (VHSV) replicated in the cultured fin cells as demonstrated by fin cell lysis, posihve immunofluorescence with polyclonal antiVHSV, and Increase in titre as measured by either infection of f ~ s h cell lines or by sandwich ELISA with anti-...
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Mammalian cytotoxic T cells as part of the adaptive immune system recognize virus-infected target cells by binding of their T-cell receptors (TCR) to classical MHC class I molecules loaded with viral peptides. Our previous studies have shown that the allele of the single dominant polymorphic classical MHC class I locus Onmy-UBA is identical in the rainbow trout clone C25 and in the permanent ra...
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متن کاملComparison of immunohistochemistry and virus cultivation for detection of viral haemorrhagic septicaemia virus in experimentally infected rainbow trout Oncorhynchus mykiss
lmmunohistochem~stry and virus isolation were compared for their ability to detect viral haemorrhagic septicaemia vlrus (VHS) in experimentally ~nfected ralnbow trout Oncorhynchus mykiss. The f ~ s h were divided into 3 g]-oups ( I to I l l ) , and infected, respectively, by bath challenge w ~ t h 102, 103 ', and 10"CID50 ml-l water of a VHS virus strain serologically sllnilar to reference stra...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2011
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao02298