Viral load of various tissues of rainbow trout challenged with viral haemorrhagic septicaemia virus at various stages of disease

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Viral load of various tissues of rainbow trout challenged with viral haemorrhagic septicaemia virus at various stages of disease.

Market-sized rainbow trout Oncorhynchus mykiss were challenged by waterborne exposure to viral haemorrhagic septicaemia virus (VHSV isolate of genogroup Ia). Fish were sampled at 4 stages of infection (before onset of clinical signs, clinically affected fish, mortalities and survivors) and the viral load determined in (1) internal organs, (2) muscle tissue and (3) brain and gill tissue. Virus l...

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In vitro susceptibility of rainbow trout fin cells to viral haemorrhagic septicaemia virus

Cells isolated from rainbow trout fins Oncorhynchus mykiss, Walbaum were propagated and subcultured a s fibroblast-like. Viral haemorrhagic septicaemia virus (VHSV) replicated in the cultured fin cells as demonstrated by fin cell lysis, posihve immunofluorescence with polyclonal antiVHSV, and Increase in titre as measured by either infection of f ~ s h cell lines or by sandwich ELISA with anti-...

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Cell-mediated cytotoxicity in rainbow trout, Oncorhynchus mykiss, infected with viral haemorrhagic septicaemia virus.

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Virulence of viral haemorrhagic septicaemia virus (VHSV) genotype III in rainbow trout.

In general, viral haemorrhagic septicaemia virus (VHSV) isolates from marine fish species in European waters (genotypes GIb, GII and GIII) are non- to low virulent in rainbow trout. However, a VHSV isolation was made in 2007 from a disease outbreak in sea farmed rainbow trout in Norway. The isolate, named NO-2007-50-385, was demonstrated to belong to GIII. This isolate has attracted attention t...

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Comparison of immunohistochemistry and virus cultivation for detection of viral haemorrhagic septicaemia virus in experimentally infected rainbow trout Oncorhynchus mykiss

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ژورنال

عنوان ژورنال: Diseases of Aquatic Organisms

سال: 2011

ISSN: 0177-5103,1616-1580

DOI: 10.3354/dao02298